Journal: Cell Death Discovery
Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy
doi: 10.1038/s41420-025-02694-4
Figure Lengend Snippet: A Fluorescence microscope was employed for detecting the miRNA-125a-5p agomir transfection efficiency in mice with liver fibrosis, B qRT-PCR was carried out for evaluating the miRNA-125a-5p agomir transfection efficiency in mice with liver fibrosis. ** p < 0.01.
Article Snippet: After 4% paraformaldehyde fixation, DAPI was added for 10 min of nuclear staining, and a positive fluorescence microscope (Axio Imager D2, Carl Zeiss, Germany) was utilized to observe the sections and take photos.
Techniques: Fluorescence, Microscopy, Transfection, Quantitative RT-PCR